DNA purification is an important step in the sample preparation workflow that removes enzymes, salts and other contaminants from lysed samples or PCR products prior to subsequent applications such as cloning or sequencing. It also eliminates unwanted PCR artifacts like primer dimers or nucleotides that are not incorporated. DNA purification in molecular biological research is a crucial step that requires careful planning to ensure quality, reliable results.

Purifying DNA can be accomplished by a variety of methods. The conventional methods for DNA isolation comprise a variety of steps, such as leukocyte separation or red blood cell lysis, to remove inhibitors of heme protein of the PCR reaction. They also include deproteinization, treatment of RNAse and precipitation of isopropanol and alcohol, and finally DNA elimination. These procedures require specialized equipment, like an electrophoresis and biosafety cabinets due to the intercalating dyes that are used in electrophoresis gels.

Other methods of DNA purification use spin columns or plates with 96 wells that separate the contaminated particles by adsorbing to the surface. These methods can be lengthy especially when you are working with a large number of samples or if the columns have to be manually refilled.

Dipsticks decrease the number of sample processing steps from six to three. They bind nucleic acid using the cellulose-based waxy material and release them when in contact with water. This method is particularly beneficial for low-resource settings such as remote http://www.mpsciences.com/2021/04/15/gene-synthesis-and-transcription-processes/ sites and teaching labs. Its simplicity and speed (30 seconds for each sample) makes it ideally suited for diagnostic molecular applications such as the detection of disease, genotype screening, and heterozygosity testing.